Chromatin Reorganization during Myoblast Differentiation Involves the Caspase-Dependent Removal of SATB2.

ABSTRACT: The induction of lineage-specific gene programs are strongly influenced by alterations in local chromatin architecture. However, key players that impact this genome reorganization remain largely unknown. Here, we report that the removal of the special AT-rich binding protein 2 (SATB2), a nuclear protein known to bind matrix attachment regions, is a key event in initiating myogenic differentiation. The deletion of myoblast SATB2 in vitro initiates chromatin remodeling and accelerates differentiation, which is dependent on the caspase 7-mediated cleavage of SATB2. A genome-wide analysis indicates that SATB2 binding within chromatin loops and near anchor points influences both loop and sub-TAD domain formation. Consequently, the chromatin changes that occur with the removal of SATB2 lead to t

SUBMITTER: Bell RAV 

PROVIDER: S-EPMC8946544 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature