Metabolic Reprogramming of Ovarian Cancer Spheroids during Adhesion.

ABSTRACT: Ovarian cancer remains a deadly disease and its recurrence disease is due in part to the presence of disseminating ovarian cancer aggregates not removed by debulking surgery. During dissemination in a dynamic ascitic environment, the spheroid cells' metabolism is characterized by low respiration and fragmented mitochondria, a metabolic phenotype that may not support secondary outgrowth after adhesion. Here, we investigated how adhesion affects cellular respiration and substrate utilization of spheroids mimicking early stages of secondary metastasis. Using different glucose and oxygen levels, we investigated cellular metabolism at early time points of adherence (24 h and less) comparing slow and fast-developing disease models. We found that adhesion over time showed changes in cellular ener

SUBMITTER: Compton SLE 

PROVIDER: S-EPMC8946790 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature