Oxidative Stress Differentially Influences the Survival and Metabolism of Cells in the Melanoma Microenvironment.

ABSTRACT: The cellular composition of the tumor microenvironment, including tumor, immune, stromal, and endothelial cells, significantly influences responses to cancer therapies. In this study, we analyzed the impact of oxidative stress, induced by cold atmospheric plasma (CAP), on tumor cells, T cells, and macrophages, which comprise part of the melanoma microenvironment. To accomplish this, cells were grown in different in vitro cell culture models and were treated with varying amounts of CAP. Subsequent alterations in viability, proliferation, and phenotype were analyzed via flow cytometry and metabolic alterations by Seahorse Cell Mito Stress Tests. It was found that cells generally exhibited reduced viability and proliferation, stemming from CAP induced G2/M cell cycle arrest and subsequent apo

SUBMITTER: Trzeciak ER 

PROVIDER: S-EPMC8946823 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature