3D In Vitro Platform for Cell and Explant Culture in Liquid-like Solids.

ABSTRACT: Existing 3D cell models and technologies have offered tools to elevate cell culture to a more physiologically relevant dimension. One mechanism to maintain cells cultured in 3D is by means of perfusion. However, existing perfusion technologies for cell culture require complex electronic components, intricate tubing networks, or specific laboratory protocols for each application. We have developed a cell culture platform that simply employs a pump-free suction device to enable controlled perfusion of cell culture media through a bed of granular microgels and removal of cell-secreted metabolic waste. We demonstrated the versatile application of the platform by culturing single cells and keeping tissue microexplants viable for an extended period. The human cardiomyocyte AC16 cell line culture

SUBMITTER: Nguyen DT 

PROVIDER: S-EPMC8946834 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature