Dataset Information

Raman Spectroscopy: In Vivo Application for Bone Evaluation in Oral Reconstructive (Regenerative) Surgery.

ABSTRACT: The aim of this study was to evaluate the quality of the bone, revealing the different phases for calcified tissues independent of the medical history of the patient in relation to periodontitis by means of in vivo Raman spectroscopy. Raman spectroscopy measurements were performed in vivo during surgery and then ex vivo for the harvested bone samples for the whole group of patients (ten patients). The specific peaks for the Raman spectrum were traced for reference compounds (e.g., calcium phosphates) and bone samples. The variation in the intensity of the spectrum in relation to the specific bone constituents' concentrations reflects the bone quality and can be strongly related with patient medical status (before dental surgery and after a healing period). Moreover, bone sample fluorescence is related to collagen content, enabling a complete evaluation of bone quality including a "quasi-quantification" of the healing process similar to the bone augmentation procedure. A complete evaluation of the processed spectra offers quantitative/qualitative information on the condition of the bone tissue. We conclude that Raman spectroscopy can be considered a viable investigation method for an in vivo and quick bone quality assessment during oral and periodontal surgery.

SUBMITTER: Gatin EG

PROVIDER: S-EPMC8947687 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Raman Spectroscopy: In Vivo Application for Bone Evaluation in Oral Reconstructive (Regenerative) Surgery.

Gatin Eduard Gheorghe EG Nagy Pal P Iordache Stefan-Marian SM Iordache Ana-Maria AM Luculescu Catalin Romeo CR

Diagnostics (Basel, Switzerland) 20220316 3

The aim of this study was to evaluate the quality of the bone, revealing the different phases for calcified tissues independent of the medical history of the patient in relation to periodontitis by means of in vivo Raman spectroscopy. Raman spectroscopy measurements were performed in vivo during surgery and then ex vivo for the harvested bone samples for the whole group of patients (ten patients). The specific peaks for the Raman spectrum were traced for reference compounds (e.g., calcium phosph ...[more]

PMID: 35328277

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Project description:SignificanceFor most patients with pituitary adenomas, surgical resection represents a viable therapeutic option, particularly in cases with endocrine symptoms or local mass effects. Diagnostic imaging, including MRI and computed tomography, is employed clinically to plan pituitary adenoma surgery. However, these methods cannot provide surgical guidance information in real time to improve resection rates and reduce risks of damage to normal tissue during tumor debulking.AimHere, we present the development of a handheld Raman spectroscopy system that can be seamlessly integrated with transsphenoidal surgery workflows to allow live discrimination of all normal intracranial anatomical structures, including the pituitary gland, and potentially tissue abnormalities such as adenomas.ApproachA fiber-optic probe was developed with a form factor compatible with endoscopic systems for endonasal surgeries. The instrument was evaluated in an ex vivo experimental protocol designed to assess its ability to distinguish normal intracranial structures. A total of 274 in situ spectroscopic measurements were acquired from six lamb heads, targeting key anatomical structures encountered in surgery. Support vector machine models were developed to classify tissue types based on their spectral signatures.ResultsBinary classification models successfully distinguished the pituitary gland from other tissue structures with a sensitivity and a specificity of 100%. In addition, a four-class predictive model enabled >95% accuracy in situ discrimination of four structures of most importance during pituitary adenoma tumor resection, i.e., the pituitary gland, the sella turcica (ST) bone, the optic chiasm, and the ST dura mater.ConclusionsThis work sets the stage for the clinical deployment of Raman spectroscopy as an intraoperative real-time decision support system during transsphenoidal surgery, with future work focused on clinical integration and the generalization of the approach to include the detection of tissue abnormalities, such as pituitary adenomas.

Dataset Information

Raman Spectroscopy: In Vivo Application for Bone Evaluation in Oral Reconstructive (Regenerative) Surgery.

Publications

Raman Spectroscopy: In Vivo Application for Bone Evaluation in Oral Reconstructive (Regenerative) Surgery.

Similar Datasets

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