Oxidative stress facilitates exogenous mitochondria internalization and survival in retinal ganglion precursor-like cells.

ABSTRACT: Ocular cells are highly dependent on mitochondrial function due to their high demand of energy supply and their constant exposure to oxidative stress. Indeed, mitochondrial dysfunction is highly implicated in various acute, chronic, and genetic disorders of the visual system. It has recently been shown that mitochondrial transplantation (MitoPlant) temporarily protects retinal ganglion cells (RGCs) from cell death during ocular ischemia. Here, we characterized MitoPlant dynamics in retinal ganglion precursor-like cells, in steady state and under oxidative stress. We developed a new method for detection of transplanted mitochondria using qPCR, based on a difference in the mtDNA sequence of C57BL/6 and BALB/c mouse strains. Using this approach, we show internalization of exogenous mitochondr

SUBMITTER: Aharoni-Simon M 

PROVIDER: S-EPMC8948238 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature