Role of detrusor PDGFRα⁺ cells in mouse model of cyclophosphamide-induced detrusor overactivity.

ABSTRACT: Cyclophosphamide (CYP)-induced cystitis is a rodent model that shares many features common to the cystitis occurring in patients, including detrusor overactivity (DO). Platelet-derived growth factor receptor alpha positive (PDGFRα⁺) cells have been proposed to regulate muscle excitability in murine bladders during filling. PDGFRα⁺ cells express small conductance Ca²⁺-activated K⁺ channels (predominantly SK3) that provide stabilization of membrane potential during filling. We hypothesized that down-regulation of the regulatory functions of PDGFRα⁺ cells and/or loss of PDGFRα⁺ cells generates the DO in CYP-treated mice. After CYP treatment, transcripts of Pdgfrα and Kcnn3 and PDGFRα and SK3 protein were reduced in detrusor mus

SUBMITTER: Lee H 

PROVIDER: S-EPMC8948241 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature