Expression of cardiovascular-related microRNAs is altered in L-arginine:glycine amidinotransferase deficient mice.

ABSTRACT: In humans and mice, L-arginine:glycine amidinotransferase (AGAT) and its metabolites homoarginine (hArg) and creatine have been linked to cardiovascular disease (CVD), specifically myocardial infarction (MI) and heart failure (HF). The underlying molecular and regulatory mechanisms, however, remain unclear. To identify potential pathways of cardiac AGAT metabolism, we sequenced microRNA (miRNA) in left ventricles of wild-type (wt) compared to AGAT-deficient (AGAT^-/-) mice. Using literature search and validation by qPCR, we identified eight significantly regulated miRNAs in AGAT^-/- mice linked to atherosclerosis, MI and HF: miR-30b, miR-31, miR-130a, miR-135a, miR-148a, miR-204, miR-298, and let-7i. Analysis of Gene Expression Omnibus (GEO) data confirmed deregulation

SUBMITTER: Jensen M 

PROVIDER: S-EPMC8948300 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature