Fish Capsules: A System for High-Throughput Screening of Combinatorial Drugs.

ABSTRACT: Large-scale screening of molecules heavily relies on phenotyping of small living organisms during preclinical development. However, deep profiling candidate therapeutics on whole animals typically requires laborious manipulations and anesthetic treatment using traditional techniques or automated tools. Here, a novel fish capsule system that combines automated zebrafish encapsulating technology and droplet microarray strategy for in vivo functional screening of mono/polytherapies is described. This platform enables automated, rapid zebrafish orientation and immobilization in agarose to generate large-scale fish capsules by using a microfluidic device. Based on the effect of discontinuous dewetting, the prompt trapping of fish capsules in the aqueous arrays is successfully demonstrate. This

SUBMITTER: Tang M 

PROVIDER: S-EPMC8948576 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature