Human C1q Regulates Influenza A Virus Infection and Inflammatory Response via Its Globular Domain.

ABSTRACT: The Influenza A virus (IAV) is a severe respiratory pathogen. C1q is the first subcomponent of the complement system's classical pathway. C1q is composed of 18 polypeptide chains. Each of these chains contains a collagen-like region located at the N terminus, and a C-terminal globular head region organized as a heterotrimeric structure (ghA, ghB and ghC). This study was aimed at investigating the complement activation-independent modulation by C1q and its individual recombinant globular heads against IAV infection. The interaction of C1q and its recombinant globular heads with IAV and its purified glycoproteins was examined using direct ELISA and far-Western blotting analysis. The effect of the complement proteins on IAV replication kinetics and immune modulation was assessed by qPCR. The

SUBMITTER: Varghese PM 

PROVIDER: S-EPMC8949502 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature