Research on Rapid Detection Technology for β₂-Agonists: Multi-Residue Fluorescence Immunochromatography Based on Dimeric Artificial Antigen.

ABSTRACT: To detect two types of β2-agonist residues at the same time, we coupled two haptens of clenbuterol (CLE) and ractopamine (RAC) to the same carrier protein through diazotization to prepare dimeric artificial antigen, and a fluorescent lateral flow immunoassay method based on europium nanoparticles (EuNP-FLFIA) was established by combining polyclonal antibodies with europium nanoparticles to form probes. Under optimized conditions, the EuNP-FLFIA could simultaneously detect eight aniline-type and one phenol-type β2-agonists, and the limits of detection (LOD) were 0.11−0.19 ng/mL and 0.12 ng/mL, respectively. The recovery rate of this method was 84.00−114.00%. This method was verified by liquid chromatography−tandem mass spectrometry (LC-MS/MS), and the test results were consistent (R2 > 0.98

SUBMITTER: Liu M 

PROVIDER: S-EPMC8949518 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature