Identification of Genomic Safe Harbors in the Anhydrobiotic Cell Line, Pv11.

ABSTRACT: Genomic safe harbors (GSHs) provide ideal integration sites for generating transgenic organisms and cells and can be of great benefit in advancing the basic and applied biology of a particular species. Here we report the identification of GSHs in a dry-preservable insect cell line, Pv11, which derives from the sleeping chironomid, Polypedilum vanderplanki, and similar to the larvae of its progenitor species exhibits extreme desiccation tolerance. To identify GSHs, we carried out genome analysis of transgenic cell lines established by random integration of exogenous genes and found four candidate loci. Targeted knock-in was performed into these sites and the phenotypes of the resulting transgenic cell lines were examined. Precise integration was achieved for three candidate GSHs, and

SUBMITTER: Miyata Y 

PROVIDER: S-EPMC8949610 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature