High-Throughput UHPLC-MS to Screen Metabolites in Feces for Gut Metabolic Health.

ABSTRACT: Feces are the product of our diets and have been linked to diseases of the gut, including Chron's disease and metabolic diseases such as diabetes. For screening metabolites in heterogeneous samples such as feces, it is necessary to use fast and reproducible analytical methods that maximize metabolite detection. As sample preparation is crucial to obtain high quality data in MS-based clinical metabolomics, we developed a novel, efficient and robust method for preparing fecal samples for analysis with a focus in reducing aliquoting and detecting both polar and non-polar metabolites. Fecal samples (n = 475) from patients with alcohol-related liver disease and healthy controls were prepared according to the proposed method and analyzed in an UHPLC-QQQ targeted platform in order to obtain a qua

SUBMITTER: Zawadzki A 

PROVIDER: S-EPMC8950041 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature