Inflorescence Transcriptome Sequencing and Development of New EST-SSR Markers in Common Buckwheat (<i>Fagopyrum esculentum</i>).

ABSTRACT: Common buckwheat (Fagopyrum esculentum M.) is known for its adaptability, good nutrition, and medicinal and health care value. However, genetic studies of buckwheat have been hindered by limited genomic resources and genetic markers. In this study, Illumina HiSeq 4000 high-throughput sequencing technology was used to sequence the transcriptome of green-flower common buckwheat (Gr) with coarse pedicels and white-flower Ukrainian daliqiao (UD) with fine pedicels. A total of 118,448 unigenes were obtained, with an average length of 1248 bp and an N50 of 1850 bp. A total of 39,432 differentially expressed genes (DEGs) were identified, and the DEGs of the porphyrins and chlorophyll metabolic pathway had significantly upregulated expression in Gr. Then, a total of 17,579 sequences contain

SUBMITTER: Liu Y 

PROVIDER: S-EPMC8950064 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature