The Human Induced Pluripotent Stem Cell Test as an Alternative Method for Embryotoxicity Testing.

ABSTRACT: The evaluation of substances for their potency to induce embryotoxicity is controlled by safety regulations. Test guidelines for reproductive and developmental toxicity rely mainly on animal studies, which make up the majority of animal usage in regulatory toxicology. Therefore, there is an urgent need for alternative in vitro methods to follow the 3R principles. To improve human safety, cell models based on human cells are of great interest to overcome species differences. Here, human induced pluripotent stem cells (hiPSCs) are an ideal cell source as they largely recapitulate embryonic stem cells without bearing ethical concerns and they are able to differentiate into most cell types of the human body. Here, we set up and characterized a fetal bovine serum (FBS)-free hiPSC-based in vitro

SUBMITTER: Galanjuk S 

PROVIDER: S-EPMC8950674 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature