Combinatorial antigen targeting strategies for acute leukemia: application in myeloid malignancy.

ABSTRACT:

Background aims

Efforts to safely and effectively treat acute myeloid leukemia (AML) by targeting a single leukemia-associated antigen with chimeric antigen receptor (CAR) T cells have met with limited success, due in part to heterogeneous expression of myeloid antigens. The authors hypothesized that T cells expressing CARs directed toward two different AML-associated antigens would eradicate tumors and prevent relapse.

Methods

For co-transduction with the authors' previously optimized CLL-1 CAR currently in clinical study (NCT04219163), the authors generated two CARs targeting either CD123 or CD33. The authors then tested the anti-tumor activity of T cells expressing each of the three CARs either alone or after co-transduction. The authors analyzed CAR T-cell phenotype, exp

SUBMITTER: Atilla PA 

PROVIDER: S-EPMC8950815 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature