Sunflower WRINKLED1 Plays a Key Role in Transcriptional Regulation of Oil Biosynthesis.

ABSTRACT: Sunflower (Helianthus annuus) is one of the most important oilseed crops worldwide. However, the transcriptional regulation underlying oil accumulation in sunflower is not fully understood. WRINKLED1 (WRI1) is an essential transcription factor governing oil accumulation in plant cells. Here, we identify and characterize a sunflower ortholog of WRI1 (HaWRI1), which is highly expressed in developing seeds. Transient production of HaWRI1 stimulated substantial oil accumulation in Nicotiana benthamiana leaves. Dual-luciferase reporter assay, electrophoretic mobility shift assay, fatty acid quantification, and gene expression analysis demonstrate that HaWRI1 acts as a pivotal transcription factor controlling the expression of genes involved in late glycolysis and fat

SUBMITTER: Lim ARQ 

PROVIDER: S-EPMC8951541 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature