Hepatocyte Proteome Alterations Induced by Individual and Combinations of Common Free Fatty Acids.

ABSTRACT: Non-alcoholic fatty liver disease is a pathology with a hard-to-detect onset and is estimated to be present in a quarter of the adult human population. To improve our understanding of the development of non-alcoholic fatty liver disease, we treated a human hepatoma cell line model, HepG2, with increasing concentrations of common fatty acids, namely myristic, palmitic and oleic acid. To reproduce more physiologically representative conditions, we also included combinations of these fatty acids and monitored the cellular response with an in-depth proteomics approach and imaging techniques. The two saturated fatty acids initially presented a similar phenotype of a dose-dependent decrease in growth rates and impaired lipid droplet formation. Detailed analysis revealed that the drop in the grow

SUBMITTER: Gindlhuber J 

PROVIDER: S-EPMC8951603 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature