KOREF_S1: phased, parental trio-binned Korean reference genome using long reads and Hi-C sequencing methods.

ABSTRACT:

Background

KOREF is the Korean reference genome, which was constructed with various sequencing technologies including long reads, short reads, and optical mapping methods. It is also the first East Asian multiomic reference genome accompanied by extensive clinical information, time-series and multiomic data, and parental sequencing data. However, it was still not a chromosome-scale reference. Here, we updated the previous KOREF assembly to a new chromosome-level haploid assembly of KOREF, KOREF_S1v2.1. Oxford Nanopore Technologies (ONT) PromethION, Pacific Biosciences HiFi-CCS, and Hi-C technology were used to build the most accurate East Asian reference assembled so far.

Results

We produced 705 Gb ONT reads and 114 Gb Pacific Biosciences HiFi reads, and corrected ONT reads

SUBMITTER: Kim HS 

PROVIDER: S-EPMC8952264 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature