Functional Classification and Characterization of the Fungal Glycoside Hydrolase 28 Protein Family.

ABSTRACT: Pectin is a major constituent of the plant cell wall, comprising compounds with important industrial applications such as homogalacturonan, rhamnogalacturonan and xylogalacturonan. A large array of enzymes is involved in the degradation of this amorphous substrate. The Glycoside Hydrolase 28 (GH28) family includes polygalacturonases (PG), rhamnogalacturonases (RG) and xylogalacturonases (XG) that share a structure of three to four pleated β-sheets that form a rod with the catalytic site amidst a long, narrow groove. Although these enzymes have been studied for many years, there has been no systematic analysis. We have collected a comprehensive set of GH28 encoding sequences to study their evolution in fungi, directed at obtaining a functional classification, as well as at the identificatio

SUBMITTER: Villarreal F 

PROVIDER: S-EPMC8952511 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature