MinION, a portable long-read sequencer, enables rapid vaginal microbiota analysis in a clinical setting.

ABSTRACT:

Background

It has been suggested that the local microbiota in the reproductive organs is relevant to women's health and may also affect pregnancy outcomes. Analysis of partial 16S ribosomal RNA (rRNA) gene sequences generated by short-read sequencers has been used to identify vaginal and endometrial microbiota, but it requires a long time to obtain the results, making it unsuitable for rapid bacterial identification from a small specimen amount in a clinical context.

Methods

We developed a simple workflow using the nanopore sequencer MinION that allows high-resolution and rapid differentiation of vaginal microbiota. Vaginal samples collected from 18 participants were subjected to DNA extraction and full-length 16S rRNA gene sequencing with MinION.

Results

The principa

SUBMITTER: Komiya S 

PROVIDER: S-EPMC8953062 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature