Quantitative super-resolution microscopy reveals promoting mitochondrial interconnectivity protects against AKI.

ABSTRACT:

Background

The root of many kidney diseases in humans can be traced to alterations or damage to subcellular organelles. Mitochondrial fragmentation, endoplasmic reticulum (ER) stress, and lysosomal inhibition, among others, ultimately contribute to kidney injury and are the target of therapeutics in development. Although recent technological advancements allow for the understanding of disease states at the cellular level, investigating changes in subcellular organelles from kidney tissue remains challenging.

Methods

Using structured illumination microscopy, we imaged mitochondria and other organelles from paraffin sections of mouse tissue and human kidney biopsy specimens. The resulting images were 3D rendered to quantify mitochondrial size, content, and morphology. Results

SUBMITTER: Taguchi K 

PROVIDER: S-EPMC8953106 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature