Monocytes contribute to a pro-healing response in 40 μm diameter uniform-pore, precision-templated scaffolds.

ABSTRACT: Porous precision-templated scaffolds (PTS) with uniformly distributed 40 μm spherical pores have shown a remarkable ability in immunomodulating resident cells for tissue regeneration. While the pore size mediated pro-healing response observed only in 40 μm pore PTS has been attributed to selective macrophage polarization, monocyte recruitment and phenotype have largely been uncharacterized in regulating implant outcome. Here, we employ a double transgenic mouse model for myeloid characterization and a multifaceted phenotyping approach to quantify monocyte dynamics within subcutaneously implanted PTS. Within 40 μm PTS, myeloid cells were found to preferentially infiltrate into the scaffold. Additionally, macrophage receptor with collagenous structure (MARCO), an innate activation marker, wa

SUBMITTER: Chan NR 

PROVIDER: S-EPMC8953147 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature