Alternative Hapten Design for Zearalenone Immunoreagent Generation.

ABSTRACT: Appropriate hapten design and synthesis have been identified as critical steps to generate high-performance immunoreagents and to develop sensitive and selective immunoanalytical methods. Antibodies and immunoassays for the major mycotoxin zearalenone have been reported and marketed. However, zearalenone haptens have mostly been prepared by the oxime active ester technique, and hapten characterization has generally been poor or non-existent. In the present study, novel haptens of zearalenone with longer linkers and with alternative tethering sites have been designed for immunizing and assay conjugate preparation. All of these molecules were purified and spectroscopically verified, and a structure-activity relationship evaluation was carried out. This approach revealed that the hapten with

SUBMITTER: Abad-Fuentes A 

PROVIDER: S-EPMC8953469 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature