Dataset Information

Mutation breeding of Aspergillus niger by atmospheric room temperature plasma to enhance phosphorus solubilization ability.

ABSTRACT:

Background

Phosphorus (P) is abundant in soils, including organic and inorganic forms. Nevertheless, most of P compounds cannot be absorbed and used by plants. Aspergillus niger v. Tiegh is a strain that can efficiently degrade P compounds in soils.

Methods

In this study, A. niger xj strain was mutated using Atmospheric Room Temperature Plasma (ARTP) technology and the strains were screened by Mo-Sb Colorimetry with strong P-solubilizing abilities.

Results

Compared with the A. niger xj strain, setting the treatment time of mutagenesis to 120 s, four positive mutant strains marked as xj 90-32, xj120-12, xj120-31, and xj180-22 had higher P-solubilizing rates by 50.3%, 57.5%, 55.9%, and 61.4%, respectively. Among them, the xj120-12 is a highly efficient P solubilizing and growth-promoting strain with good application prospects. The growth characteristics such as plant height, root length, and dry and fresh biomass of peanut (Arachis hypogaea L.) increased by 33.5%, 43.8%, 43.4%, and 33.6%, respectively. Besides available P, the chlorophyll and soluble protein contents also vary degrees of increase in the P-solubilizing mutant strains.

Conclusions

The results showed that the ARTP mutagenesis technology can improve the P solubilization abilities of the A. niger mutant strains and make the biomass of peanut plants was enhanced of mutant strains.

SUBMITTER: Peng Q

PROVIDER: S-EPMC8953557 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Publications

Mutation breeding of <i>Aspergillus niger</i> by atmospheric room temperature plasma to enhance phosphorus solubilization ability.

Peng Qiuju Q Xiao Yang Y Zhang Su S Zhou Changwei C Xie Ailin A Li Zhu Z Tan Aijuan A Zhou Lihong L Xie Yudan Y Zhao Jinyi J Wu Chenglin C Luo Lei L Huang Jie J He Tengxia T Sun Ran R

PeerJ 20220322

<h4>Background</h4>Phosphorus (P) is abundant in soils, including organic and inorganic forms. Nevertheless, most of P compounds cannot be absorbed and used by plants. <i>Aspergillus niger</i> v. Tiegh is a strain that can efficiently degrade P compounds in soils.<h4>Methods</h4>In this study, <i>A. niger</i> xj strain was mutated using Atmospheric Room Temperature Plasma (ARTP) technology and the strains were screened by Mo-Sb Colorimetry with strong P-solubilizing abilities.<h4>Results</h4>Com ...[more]

PMID: 35341057

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