Retention and Fouling during Nanoparticle Filtration: Implications for Membrane Purification of Biotherapeutics.

ABSTRACT: One major challenge in the development of nanoparticle-based therapeutics, including viral vectors for the delivery of gene therapies, is the development of cost-effective purification technologies. The objective of this study was to examine fouling and retention behaviors during the filtration of model nanoparticles through membranes of different pore sizes and the effect of solution conditions. Data were obtained with 30 nm fluorescently labeled polystyrene latex nanoparticles using both cellulosic and polyethersulfone membranes at a constant filtrate flux, and both pressure and nanoparticle transmission were evaluated as a function of cumulative filtrate volume. The addition of NaCl caused a delay in nanoparticle transmission and an increase in fouling. Nanoparticle transmission was als

SUBMITTER: Chu LK 

PROVIDER: S-EPMC8953984 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature