Genome-Wide Association Study Reveals Host Factors Affecting Conjugation in Escherichia coli.

ABSTRACT: The emergence and dissemination of antibiotic resistance threaten the treatment of common bacterial infections. Resistance genes are often encoded on conjugative elements, which can be horizontally transferred to diverse bacteria. In order to delay conjugative transfer of resistance genes, more information is needed on the genetic determinants promoting conjugation. Here, we focus on which bacterial host factors in the donor assist transfer of conjugative plasmids. We introduced the broad-host-range plasmid pKJK10 into a diverse collection of 113 Escherichia coli strains and measured by flow cytometry how effectively each strain transfers its plasmid to a fixed E. coli recipient. Differences in conjugation efficiency of up to 2.7 and 3.8 orders of magnitude were observed afte

SUBMITTER: Van Wonterghem L 

PROVIDER: S-EPMC8954029 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature