Antibodies to biotin enable large-scale detection of biotinylation sites on proteins.

ABSTRACT: Although purification of biotinylated molecules is highly efficient, identifying specific sites of biotinylation remains challenging. We show that anti-biotin antibodies enable unprecedented enrichment of biotinylated peptides from complex peptide mixtures. Live-cell proximity labeling using APEX peroxidase followed by anti-biotin enrichment and mass spectrometry yielded over 1,600 biotinylation sites on hundreds of proteins, an increase of more than 30-fold in the number of biotinylation sites identified compared to streptavidin-based enrichment of proteins.

SUBMITTER: Udeshi ND 

PROVIDER: S-EPMC8954634 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature