Decrease in ovarian reserve through the inhibition of SIRT1-mediated oxidative phosphorylation.

ABSTRACT:

Objective

To establish an oxidative stress-induced model of premature ovarian insufficiency (POI) and to explore the effect of SIRT1 and mitochondrial oxidative phosphorylation on the ovarian reserve.

Methods

Mice were treated with intraperitoneal injections of 3-nitropropionic acid (3-NPA) at different doses and for different time periods to induce a model of POI. Subsequently, the efficiency of each regimen was evaluated. The expression of SIRT1 in ovarian tissue was examined. Then, SIRT1 was knocked down in human luteinized granulosa cells (GCs), and its function and related receptor and gene expression were examined. Finally, a SIRT1 antagonist and agonist were used to explore the effects of SIRT1 on ovarian function in vivo and on the change in mitochondrial oxid

SUBMITTER: Guo L 

PROVIDER: S-EPMC8954953 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature