Compositional and toxicological investigation of pooled venom from farm-raised Naja atra.

ABSTRACT:

Background

Naja atra is a venomous snake species medically relevant in China. In the current study, we evaluated the composition and toxicological profile of venom collected from farm-raised N. atra.

Methods

Venom was collected from third-generation captive bred N. atra on a snake farm in Hunan Province, China. The venom was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis and nano-liquid chromatography with electrospray ionization tandem mass spectrometry. In addition, hemolytic activity, median lethal dose, serum biochemical and histopathological parameters were accessed.

Results

N. atra venom proteome was dominated by phospholipase A₂ (46.5%) and three-finger toxins (41.4 %), and a set of common low rel

SUBMITTER: Xiao G 

PROVIDER: S-EPMC8956250 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature