M⁶A mRNA modification maintains colonic epithelial cell homeostasis via NF-κB-mediated antiapoptotic pathway.

ABSTRACT: Colonic mucosal barrier dysfunction is one of the major causes of inflammatory bowel disease (IBD). However, the mechanisms underlying mucosal barrier dysfunction are poorly understood. N⁶-methyladenosine (m⁶A) mRNA modification is an important modulator of epitranscriptional regulation of gene expression, participating in multiple physiological and pathological processes. However, the function of m⁶A modification in colonic epithelial cells and stem cells is unknown. Here, we show that m⁶A modification is essential for maintaining the homeostatic self-renewal in colonic stem cells. Specific deletion of the methyltransferase 14 (Mettl14) gene in mouse colon resulted in colonic stem cell apoptosis, causing mucosal barrier dysfunction

SUBMITTER: Zhang T 

PROVIDER: S-EPMC8956260 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature