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Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62.

ABSTRACT: The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.

SUBMITTER: Yu G

PROVIDER: S-EPMC8956640 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62.

Yu Guotai G Matny Oadi O Champouret Nicolas N Steuernagel Burkhard B Moscou Matthew J MJ Hernández-Pinzón Inmaculada I Green Phon P Hayta Sadiye S Smedley Mark M Harwood Wendy W Kangara Ngonidzashe N Yue Yajuan Y Gardener Catherine C Banfield Mark J MJ Olivera Pablo D PD Welchin Cole C Simmons Jamie J Millet Eitan E Minz-Dub Anna A Ronen Moshe M Avni Raz R Sharon Amir A Patpour Mehran M Justesen Annemarie F AF Jayakodi Murukarthick M Himmelbach Axel A Stein Nils N Wu Shuangye S Poland Jesse J Ens Jennifer J Pozniak Curtis C Karafiátová Miroslava M Molnár István I Doležel Jaroslav J Ward Eric R ER Reuber T Lynne TL Jones Jonathan D G JDG Mascher Martin M Steffenson Brian J BJ Wulff Brande B H BBH

Nature communications 20220325 1

The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to c ...[more]

PMID: 35338132

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Project description:The rust diseases, including leaf rust caused by Puccinia triticina (Pt), stem rust caused by P. graminis f. sp. tritici (Pgt), and stripe rust caused by P. striiformis f. sp. tritici (Pst), are major limiting factors in wheat production worldwide. Identification of novel sources of rust resistance genes is key to developing cultivars resistant to rapidly evolving pathogen populations. Aegilops longissima is a diploid wild grass native to the Levant and closely related to the modern bread wheat D subgenome. To explore resistance genes in the species, we evaluated a large panel of Ae. longissima for resistance to several races of Pt, Pgt, and Pst, and conducted a genome-wide association study (GWAS) to map rust resistance loci in the species. A panel of 404 Ae. longissima accessions, mostly collected from Israel, were screened for seedling-stage resistance to four races of Pt, four races of Pgt, and three races of Pst. Out of the 404 accessions screened, two were found that were resistant to all 11 races of the three rust pathogens screened. The percentage of all accessions screened that were resistant to a given rust pathogen race ranged from 18.5% to 99.7%. Genotyping-by-sequencing (GBS) was performed on 381 accessions of the Ae. longissima panel, wherein 125,343 single nucleotide polymorphisms (SNPs) were obtained after alignment to the Ae. longissima reference genome assembly and quality control filtering. Genetic diversity analysis revealed the presence of two distinct subpopulations, which followed a geographic pattern of a northern and a southern subpopulation. Association mapping was performed in the genotyped portion of the collection (n = 381) and in each subpopulation (n = 204 and 174) independently via a single-locus mixed-linear model, and two multi-locus models, FarmCPU, and BLINK. A large number (195) of markers were significantly associated with resistance to at least one of 10 rust pathogen races evaluated, nine of which are key candidate markers for further investigation due to their detection via multiple models and/or their association with resistance to more than one pathogen race. The novel resistance loci identified will provide additional diversity available for use in wheat breeding.

Dataset Information

Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62.

Publications

Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62.

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