Impact of baseline culture conditions of cancer organoids when determining therapeutic response and tumor heterogeneity.

ABSTRACT: Representative models are needed to screen new therapies for patients with cancer. Cancer organoids are a leap forward as a culture model that faithfully represents the disease. Mouse-derived cancer organoids (MDCOs) are becoming increasingly popular, however there has yet to be a standardized method to assess therapeutic response and identify subpopulation heterogeneity. There are multiple factors unique to organoid culture that could affect how therapeutic response and MDCO heterogeneity are assessed. Here we describe an analysis of nearly 3500 individual MDCOs where individual organoid morphologic tracking was performed. Change in MDCO diameter was assessed in the presence of control media or targeted therapies. Individual organoid tracking was identified to be more sensitive to treatme

SUBMITTER: DeStefanis RA 

PROVIDER: S-EPMC8956720 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature