Ratiometric Imaging of Mitochondrial Hydrogen Peroxide in Aβ₄₂-Mediated Neurotoxicity.

ABSTRACT: Oxidative stress is important for the etiology and pathogenesis of Alzheimer's disease (AD). Research tools that can conveniently evaluate oxidative stress in AD models are expected to catalyze and accelerate research on AD. This study explored the use of genetically encoded fluorescent indicators (GEFIs) to detect mitochondrial oxidative stress in organotypic brain slices and AD mouse models. To enable ratiometric normalization and avoid tissue autofluorescence, we genetically fused a green fluorescent hydrogen peroxide (H₂O₂) indicator, HyPer7, with each of two selected, bright red fluorescent proteins (RFPs), mScarlet-I and tdTomato. The resultant indicators, namely, HyPerGR_S and HyPerGR_T, were tagged with mitochondrial targeting sequences and

SUBMITTER: Li X 

PROVIDER: S-EPMC8957525 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature