Regulation of Gramicidin Channel Function Solely by Changes in Lipid Intrinsic Curvature.

ABSTRACT: Membrane protein function is regulated by the lipid bilayer composition. In many cases the changes in function correlate with changes in the lipid intrinsic curvature (c ₀), and c ₀ is considered a determinant of protein function. Yet, water-soluble amphiphiles that cause either negative or positive changes in curvature have similar effects on membrane protein function, showing that changes in lipid bilayer properties other than c ₀ are important-and may be dominant. To further investigate the mechanisms underlying the bilayer regulation of protein function, we examined how maneuvers that alter phospholipid head groups effective "size"-and thereby c ₀-alter gramicidin (gA) channel function. Using dioleoylphospholipids

SUBMITTER: Maer AM 

PROVIDER: S-EPMC8957996 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature