Fine-tuning of epithelial taste bud organoid to promote functional recapitulation of taste reactivity.

ABSTRACT: Taste stem/progenitor cells from posterior mouse tongues have been used to generate taste bud organoids. However, the inaccessible location of taste receptor cells is observed in conventional organoids. In this study, we established a suspension-culture method to fine-tune taste bud organoids by apicobasal polarity alteration to form the accessible localization of taste receptor cells. Compared to conventional Matrigel-embedded organoids, suspension-cultured organoids showed comparable differentiation and renewal rates to those of taste buds in vivo and exhibited functional taste receptor cells and cycling progenitor cells. Accessible taste receptor cells enabled the direct application of calcium imaging to evaluate the taste response. Moreover, suspension-cultured organoids can be genetic

SUBMITTER: Adpaikar AA 

PROVIDER: S-EPMC8958342 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature