Design and fabrication of an improved dynamic flow cuvette for ¹³CO₂ labeling in Arabidopsis plants.

ABSTRACT:

Background

Stable isotope labeling is a non-invasive, sensitive means of monitoring metabolic flux in plants. The most physiologically meaningful information is obtained from experiments that take advantage of the natural photosynthetic carbon assimilation pathway to introduce a traceable marker with minimal effects on the physiology of the organism. The fundamental substrate in isotopic labeling experiments is ¹³CO₂, which can reveal the earliest events in carbon assimilation and realistically portray downstream metabolism when administered under conditions suitable for making kinetic inferences. Efforts to improve the accuracy and resolution of whole plant labeling techniques have focused on improvements in environmental control, air flow characteristics, an

SUBMITTER: Evans SE 

PROVIDER: S-EPMC8958768 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature