High-speed, cortex-wide volumetric recording of neuroactivity at cellular resolution using light beads microscopy.

ABSTRACT: Two-photon microscopy has enabled high-resolution imaging of neuroactivity at depth within scattering brain tissue. However, its various realizations have not overcome the tradeoffs between speed and spatiotemporal sampling that would be necessary to enable mesoscale volumetric recording of neuroactivity at cellular resolution and speed compatible with resolving calcium transients. Here, we introduce light beads microscopy (LBM), a scalable and spatiotemporally optimal acquisition approach limited only by fluorescence lifetime, where a set of axially separated and temporally distinct foci record the entire axial imaging range near-simultaneously, enabling volumetric recording at 1.41 × 10⁸ voxels per second. Using LBM, we demonstrate mesoscopic and volumetric imaging at multiple

SUBMITTER: Demas J 

PROVIDER: S-EPMC8958902 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature