Selective requirement for polycomb repressor complex 2 in the generation of specific hypothalamic neuronal subtypes.

ABSTRACT: The hypothalamus displays staggering cellular diversity, chiefly established during embryogenesis by the interplay of several signalling pathways and a battery of transcription factors. However, the contribution of epigenetic cues to hypothalamus development remains unclear. We mutated the polycomb repressor complex 2 gene Eed in the developing mouse hypothalamus, which resulted in the loss of H3K27me3, a fundamental epigenetic repressor mark. This triggered ectopic expression of posteriorly expressed regulators (e.g. Hox homeotic genes), upregulation of cell cycle inhibitors and reduced proliferation. Surprisingly, despite these effects, single cell transcriptomic analysis revealed that most neuronal subtypes were still generated in Eed mutants. However, we observed an increase in glutama

SUBMITTER: Yaghmaeian Salmani B 

PROVIDER: S-EPMC8959139 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature