Carboxymethyl chitosan bounded iron oxide nanoparticles and gamma-irradiated avian influenza subtype H9N2 vaccine to development of immunity on mouse and chicken.

ABSTRACT:

Background

Avian influenza virus (AIV) subtype H9N2 is a low pathogenic avian influenza virus (LPAIV).

Objective

This study aims to evaluate the humoral and cellular immunity in vaccinated mice and broiler chicken by irradiated AIV antigen plus carboxymethyl chitosan bounded iron oxide nanoparticles (CMC-IO NPs) as an adjuvant.

Methods

AIV subtype H9N2 with 10^8.5 EID₅₀ /ml and haemagglutinin antigen assay about 10 log₂ was irradiated by 30 kGy gamma radiation dose. Then, the gamma-irradiated AIV was used as an inactivated vaccine and conjugated with CMC-IO NPs to improve immune responses on mice. IO NPs must be applied in all activated tests using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysulfosuccinimide sodium

SUBMITTER: Motamedi-Sedeh F 

PROVIDER: S-EPMC8959295 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature