Beta hemolysin gene of Staphylococcus phage 3AJ_2017 genome is a suitable molecular marker for identification and characterization of pathogenic Staphylococcus aureus.

ABSTRACT:

Background

Staphylococcus aureus cause diseases both in humans and animals. These diseases range from mild to fatal infections thus necessitating development of a specific molecular method for detection of pathogenic S. aureus.

Objectives

To identify and analyze genetic profile of pathogenic S. aureus using bacteriophage based genetic biomarkers.

Methods

Using culture and biochemical methods, 148 S. aureus (87 %) were isolated from 170 raw milk samples taken from 10 dairy farms in Marsabit and Isiolo counties in Northern Kenya between June 2016 and February 2017. The samples were collected directly from dairy lactating cows previously diagnosed with S. aureus in a follow-up study. The isolates were analyzed by PCR and sequencing of beta hemolysin (hlb) gene. The genet

SUBMITTER: Miruka SA 

PROVIDER: S-EPMC8959332 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature