Anti-phospholipase A2 receptor antibodies directly induced podocyte damage in vitro.

ABSTRACT:

Background

The pathogenesis of primary membranous nephropathy (MN) involves the antibodies against antigens on the cell surface of podocytes, with the majority of M-type phospholipase A2 receptor (PLA2R), and a profound podocyte dysfunction. The effects of anti-PLA2R antibodies directly to the podocytes remain unclear.

Methods

Anti-PLA2R antibodies from patients with PLA2R-associated MN were affinity-purified using a column coupled with recombinant human PLA2R protein. Their effects on conditionally immortalized human podocytes were assessed by apoptosis assays, cellular calcium detection, wound healing assay, and immunofluorescent staining. Proteomics analysis was performed by LC-MS/MS and on PANTHER database.

Results

The stimulation by anti-PLA2R antibodies could in

SUBMITTER: Li Y 

PROVIDER: S-EPMC8959519 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature