Development of a Real-time TaqMan PCR Assay for The Detection of Porcine Circovirus 4.

ABSTRACT:

Introduction

Porcine circovirus 4 (PCV4) was first discovered in 2019 in a herd of pigs with porcine respiratory disease, dermatitis and nephropathy syndrome in Hunan Province, China. It has subsequently been detected in other provinces and in South Korea. In consideration of the potential of the virus to cause an epidemic, rapid, sensitive, and specific detection of PCV4 is needed, as is the facilitation of further epidemiological research through elucidation of the whole genome of PCV4. This study had those two aims.

Material and methods

Fifty-five blood samples, two pig tissue samples, nine saliva swabs and one semen sample which all originated from Sichuan province pig farms were analysed. The virus' genome of 1,770 bp was synthesised artificially based on a Chinese refe

SUBMITTER: Chen W 

PROVIDER: S-EPMC8959683 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature