Effect of Serial in Vivo Passages on The Adaptation of H1N1 Avian Influenza Virus To Pigs.

ABSTRACT:

Introduction

The lack of proofreading activity of the viral polymerase and the segmented nature of the influenza A virus (IAV) genome are responsible for the genetic diversity of IAVs and for their ability to adapt to a new host. We tried to adapt avian IAV (avIAV) to the pig by serial passages in vivo and assessed the occurrence of point mutations and their influence on viral fitness in the pig's body.

Material and methods

A total of 25 in vivo avIAV passages of the A/duck/Bavaria/77 strain were performed by inoculation of 50 piglets, and after predetermined numbers of passages 20 uninoculated piglets were exposed to the virus through contact with inoculated animals. Clinical signs of swine influenza were assessed daily. Nasal swabs and lung tissue were used t

SUBMITTER: Urbaniak K 

PROVIDER: S-EPMC8959685 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature