Prp19 Facilitated p21-Dependent Senescence of Hepatocellular Carcinoma Cells.

ABSTRACT:

Introduction

Evidence suggests that the role of senescence in the development of cancer is context-dependent. An orthologue of human pre-mRNA processing factor 19 (Prp19) attenuates the senescence of human endothelial cells. Prp19 has been reported to be involved in the progression of hepatocellular carcinoma (HCC). This work aims to investigate the effect of Prp19 on the senescence of HCC.

Materials and methods

Senescence of L02 cells and HCC cells under different stimuli was detected through cell cycle analysis, SA-β-gal staining, and senescence associated secretory phenotype analysis. The relationship between Prp19 and senescence-related proteins was evaluated using real-time RT-PCR, western blot assay, and immunohistochemistry. Subcutaneous xenograft tumors in nud

SUBMITTER: Yin J 

PROVIDER: S-EPMC8959953 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature