Programmable design of isothermal nucleic acid diagnostic assays through abstraction-based models.

ABSTRACT: Accelerating the design of nucleic acid amplification methods remains a critical challenge in the development of molecular tools to identify biomarkers to diagnose both infectious and non-communicable diseases. Many of the principles that underpin these mechanisms are often complex and can require iterative optimisation. Here we focus on creating a generalisable isothermal nucleic acid amplification methodology, describing the systematic implementation of abstraction-based models for the algorithmic design and application of assays. We demonstrate the simplicity, ease and flexibility of our approach using a software tool that provides amplification schemes de novo, based upon a user-input target sequence. The abstraction of reaction network predicts multiple reaction pathways across differ

SUBMITTER: Xu G 

PROVIDER: S-EPMC8960814 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature