An optimized ChIP-Seq framework for profiling histone modifications in Chromochloris zofingiensis.

ABSTRACT: The eukaryotic green alga Chromochloris zofingiensis is a reference organism for studying carbon partitioning and a promising candidate for the production of biofuel precursors. Recent transcriptome profiling transformed our understanding of its biology and generally algal biology, but epigenetic regulation remains understudied and represents a fundamental gap in our understanding of algal gene expression. Chromatin immunoprecipitation followed by deep sequencing (ChIP-Seq) is a powerful tool for the discovery of such mechanisms, by identifying genome-wide histone modification patterns and transcription factor-binding sites alike. Here, we established a ChIP-Seq framework for Chr. zofingiensis yielding over 20 million high-quality reads per sample. The most critical steps in

SUBMITTER: Strenkert D 

PROVIDER: S-EPMC8961045 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature