Characterization of Monoclonal Antibodies Recognizing Citrulline-Modified Residues.

ABSTRACT:

Background

Citrullination is a post-translational protein modification linked to the occurrence and development of a variety of diseases. The detection of citrullinated proteins is predominately based on antibody detection although currently available reagents demonstrate detection bias according to the environmental context of the citrullinated residues. This study aimed to develop improved antibody reagents capable of detecting citrullinated residues in proteins in an unbiased manner.

Methods

BALB/c mice were sequentially immunized using citrulline conjugates with different carrier proteins, and specific monoclonal antibodies (mAbs) identified by primary screening using citrulline-conjugated proteins unrelated to the immunogen. Secondary screening was performed to identify

SUBMITTER: Chen Y 

PROVIDER: S-EPMC8961739 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature