BMI1 promotes osteosarcoma proliferation and metastasis by repressing the transcription of SIK1.

ABSTRACT:

Background

Osteosarcoma (OS) is the most common malignant tumor of bone, and the clinical efficacy of current treatments and associated survival rates need to be further improved by employing novel therapeutic strategies. Although various studies have shown that BMI1 protein is universally upregulated in OS cells and tissues, its specific role and underlying mechanism have not yet been fully explored.

Methods

Expression of BMI1 protein in OS cells was detected by western blot. The effect of BMI1 on proliferation and migration of OS cells (143B and U-2OS cell lines) was investigated in vitro using CCK-8, colony formation and transwell assays, and in vivo using subcutaneous tumorigenesis and lung metastasis assays in xenograft nude mice. Expression of epithelial-mesenchymal tr

SUBMITTER: Wang Q 

PROVIDER: S-EPMC8961961 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature