Immune cell atlas of cholangiocarcinomas reveals distinct tumor microenvironments and associated prognoses.

ABSTRACT:

Background

Immunotherapy has demonstrated a limited clinical efficacy in approximately 5% of cholangiocarcinoma. The main challenges for an effective immunotherapy response in cholangiocarcinoma arise from the tumor microenvironment, which is poorly understood.

Methods

For a comprehensive analysis of the tumor microenvironment in cholangiocarcinoma, we performed multiplex immunohistochemistry with two 15-marker immune panels and Nanostring assays for a comprehensive analysis of 104 surgically resected cholangiocarcinomas including intrahepatic, hilar, and distal cholangiocarcinoma. We also validated some key findings with a batch integration analysis of published single cell RNA sequencing data.

Results

This study found that natural killer cells occupy the largest imm

SUBMITTER: Xia T 

PROVIDER: S-EPMC8962046 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature